mpo inhibitor 475944-1gm abah  (Millipore)

Journal: PLoS ONE

Article Title: Measuring Myeloperoxidase Activity in Biological Samples

doi: 10.1371/journal.pone.0067976

Figure Lengend Snippet: ( A ) MPO-Gd molecular MR imaging reveals MPO inhibition in vivo in mice with experimental autoimmune encephalomyelitis that were treated with ABAH. MPO activity maps are shown in 3D from two angles (left), as well as overlays of MPO activity maps over T1 images (right). ( B ) Quantification of imaging reveals significant difference in MPO activity in vivo ( P = 0.03, n = 8 per group). ( C ) In vitro assays on whole tissue homogenates using ADHP or TMB do not confirm the in vivo imaging finding ( P = 0.68 and 0.88, respectively, n = 4 per group). *: P <0.05, n.s. = not statistically significant. MPO = myeloperoxidase. TMB = 3,3′,5,5′-Tetramethylbenzidine. ADHP = 10-acetyl-3,7-dihydroxyphenoxazine. ABAH = 4-aminobenzoic acid hydrazide. Activation ratio = contrast-to-noise ratio 60 minutes over 15 minutes post MPO-Gd injection.

Article Snippet: To confirm the presence of MPO activity in EAE in vivo and to evaluate the effects of the specific MPO inhibitor ABAH (4-Aminobenzoic acid hydrazide, Sigma) noninvasively, we performed MPO-Gd ( bis -5-hydroxytryptamide-diethylenetriaminepentaacetate-gadolinium) molecular MR imaging in mice with EAE.

Techniques: Imaging, Inhibition, In Vivo, Activity Assay, In Vitro, In Vivo Imaging, Activation Assay, Injection

Journal: Neoplasia (New York, N.Y.)

Article Title: Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy

doi: 10.1016/j.neo.2022.100779

Figure Lengend Snippet: Semi-quantitative measurement of in-vivo MPO inhibition by MPO-Gd MRI. In vivo MPO activity, measured by bis-5-hydroxytryptamide-diethylenetriaminepentaacetate-gadolinium (MPO-Gd) imaging, in tumor-bearing mice that were either injected with saline (IR-Saline) or the MPO-inhibitor 4-Aminobenzoic acid hydrazide (IR-ABAH) at two weeks after irradiation. (A) Representative axial T1-weighted images showing early (15 min) and delayed (60 min) contrast-enhanced images acquired after MPO-Gd injection; outlines highlight tumors in the brain (B) Bar graph showing MPO activation ratio of irradiated-tumors in saline (n = 2) and ABAH (n = 3) treated mice. MPO activation ratio was computed as a ratio of the contrast-to-noise ratios (CNRs) calculated from delayed (60 min) and early (15 min) post-contrast T1-weighted images. Data plotted as mean ± SEM. *p < 0.05.

Article Snippet: To investigate the post-irradiation effects of MPO inhibition, mice were intraperitoneally injected twice daily with 40 mg/kg of specific MPO inhibitor ABAH (4- Aminobenzoic acid hydrazide, Sigma), starting one hour after irradiation until the experimental endpoint and compared with saline injected mice as controls.

Techniques: In Vivo, Inhibition, Activity Assay, Imaging, Injection, Irradiation, Activation Assay

Journal: Neoplasia (New York, N.Y.)

Article Title: Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy

doi: 10.1016/j.neo.2022.100779

Figure Lengend Snippet: MPO inhibition with ABAH decreased inflammatory cell recruitment. (A-D) Flow cytometry analysis showing neutrophil and Ly-6C high monocyte (Mo) or monocytes/macrophage (Mo/MΦ) cell numbers out of total leukocytes defined as CD45+ cells, in brain tumor (A & B), peripheral blood (C) and bone marrow (D) of irradiated mice treated either with saline (IR-Saline) or ABAH (IR-ABAH) (n = 5/group). Mice were irradiated 14 days after tumor injections and flow cytometry was performed 1 week after radiation. SSC: side scatter, Ly6G: lymphocyte antigen 6G. Ly6C: lymphocyte antigen 6C. Data plotted as mean ± SEM. *p < 0.05.

Article Snippet: To investigate the post-irradiation effects of MPO inhibition, mice were intraperitoneally injected twice daily with 40 mg/kg of specific MPO inhibitor ABAH (4- Aminobenzoic acid hydrazide, Sigma), starting one hour after irradiation until the experimental endpoint and compared with saline injected mice as controls.

Techniques: Inhibition, Flow Cytometry, Irradiation

Journal: Neoplasia (New York, N.Y.)

Article Title: Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy

doi: 10.1016/j.neo.2022.100779

Figure Lengend Snippet: MPO inhibition or absence post-radiation accelerated tumor growth. (A) Axial T1 weighted pre- and post-contrast diethylenetriaminepentaacetate-gadolinium (DTPA-Gd) enhanced images acquired from tumor bearing mice at 1 week after radiation. Experimental groups comprised of wild-type mice treated with saline (IR-Saline) or ABAH (IR-ABAH), and MPO-knock out mice (IR-MPO-KO group). (B-C) Tumor size measured as the bi-dimensional product (cm 2 ) at one week (B, n = 4/group) and two weeks (C, n = 5-9/group) after irradiation. Data plotted as mean ± SEM. *p < 0.05, **p < 0.01, ns = not significant.

Article Snippet: To investigate the post-irradiation effects of MPO inhibition, mice were intraperitoneally injected twice daily with 40 mg/kg of specific MPO inhibitor ABAH (4- Aminobenzoic acid hydrazide, Sigma), starting one hour after irradiation until the experimental endpoint and compared with saline injected mice as controls.

Techniques: Inhibition, Knock-Out, Irradiation

Journal: Neoplasia (New York, N.Y.)

Article Title: Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy

doi: 10.1016/j.neo.2022.100779

Figure Lengend Snippet: Contrasting role of MPO inhibition on animal survival in irradiated and non-irradiated mice. (A) Survival analysis of the IR-Saline (n = 15), IR-ABAH (n = 12) and IR-MPO-KO (n = 5) mice groups. *p < 0.05, **p < 0.01 (B) Survival analysis of the saline and ABAH treated mice that were not irradiated (non-IR). Twice daily saline or ABAH injections were started from day 14 after tumor implantation. n = 9/group, *p < 0.05.

Article Snippet: To investigate the post-irradiation effects of MPO inhibition, mice were intraperitoneally injected twice daily with 40 mg/kg of specific MPO inhibitor ABAH (4- Aminobenzoic acid hydrazide, Sigma), starting one hour after irradiation until the experimental endpoint and compared with saline injected mice as controls.

Techniques: Inhibition, Irradiation, Tumor Implantation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: DNA-based fluorescent probes of NOS2 activity in live brains

doi: 10.1073/pnas.2003034117

Figure Lengend Snippet: NOckoutFn detects NO in mouse primary microglia and in alveolar macrophages. (A) Representative confocal images of NOckout from LPS (1 μg/mL) primed J774A.1 macrophages in A647 (R) and DAR (G) channels in the absence (Upper) or presence (Lower) of NOS2 inhibitor 1400W. G/R intensities are represented as heat maps. (B) Representative confocal images of NOckoutmCpG from mouse primary microglia. Cells were incubated with NOckoutmCpG (500 nM) in the absence (Upper) or presence (Lower) of 1400W for 120 min in DMEM, imaged in DAR(G) and A647(R) channels, and converted into G/R heat maps. (C) Representative heat map (G/R) images of NOckout-, NOckoutiRNA-, and NOckoutRNA-treated J774A.1 cells. (D) Violin plot of the distribution of G/R values of ∼200 individual endosomes (n = 30 cells) of J774A.1 cells treated with VAS2870, ABAH, and 1400W in the presence and absence of LPS. (E) Violin plot of the distribution of G/R values of ∼100 individual endosomes (n = 20 cells) in primary microglia treated with NOckoutmCpG and NOckoutmGpC. (F) Violin plot of the distribution of G/R values of ∼200 individual endosomes (n = 30 cells) in J774A.1 macrophages treated with NOckoutRNA variants in the presence and absence of 1400W. All experiments were performed in triplicate. (Scale bar, 10 μm.) P values are obtained using Kruskal−Wallis statistical test across the dataset.

Article Snippet: Nitric oxide donor (DEANONOate), iNOS inhibitor (1400W), NADPH-oxidase inhibitor (VAS2870), MPO inhibitor (ABAH), and vacuolar H + -ATPase (V-ATPase) inhibitor (Bafilomycin A1) were purchased from Cayman Chemicals.

Techniques: Incubation